Movie S7.
NMII minifilaments coordinate peg dynamics and microridge fusion, fission, and spacing. 9-minute time-lapse videos with 1-minute intervals of periderm cells expressing fluorescent reporters for actin (Lifeact-mRuby) and NMII (Myl12.1-EGFP). NMII minifilaments appear as two green puncta. Different NMII-mediated events indicated by title cards. "Bridges" of one or two NMII minifilaments attach to pegs as they appear, and occasionally pull them toward one another. NMII minifilaments connect two microridge ends and fuse them into a longer microridge. NMII minifilaments oriented perpendicular to a microridge in the x-y plane sever a microridge. Finally, NMII minifilament "bridges" connecting two adjacent microridges contract to pull the microridges closer together, and allow microridges to drift further apart as they disappear. Scale bar: 1μm.