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. 2021 Aug 15;32(17):1523–1533. doi: 10.1091/mbc.E20-11-0728

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© 2021 Sabinina et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 2: — Average intensity map of individual Nups. Representative dual-color 9-μm² area of the lower surface of the nucleus of a U2OS cell, endogenously expressing (A) SNAP-RANBP2, (B) SNAP-SEH1, (C) SNAP-NUP107, (D) SNAP-NUP133, and (E) TPR-SNAP, respectively, labeled with BG-AF647 and with α-ELYS primary antibody and secondary coupled to CF680. Top view and side view of density maps of the 3D averages of 3D SMLM data sets (RANBP2 [n = 2140]: light blue, SEH1 [n = 1619]: red, NUP107 [n = 3617]: light green; NUP133 [n = 2418]: purple, TPR [n = 3066]: yellow and ELYS: orange). Scale bars, 1 μm for overview images and 50 nm for average intensity map.