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. 2021 Aug 15;32(17):1545–1556. doi: 10.1091/mbc.E21-02-0060

FIGURE 2:

FIGURE 2:

Effects of centrinone and blebbistatin on polyploidy and cell migration. (A) Representative histograms of propidium iodide (PI) staining for ploidy determination in centrinone-treated compared with DMSO-treated PLB-985 cells. (B) Graph showing quantification of the ploidy (data displayed as mean + SD). (C) Representative histogram of PI staining for ploidy analysis in cells treated with DMSO or 20 µM blebbistatin. Cells were treated for the first 4 days of differentiation and then treatment was washed out; cells were used on day 6. (D) Mean 2D track speed and chemotactic index of blebbistatin and DMSO-treated PLB-985 cells (data displayed as mean + SD; N = 2 repeats, n = 6 devices, 1159–2377 cells). Cells were imaged in a 10-µg/ml fibronectin-coated microfluidic device migrating toward an fMLP gradient every 30 s for 45 min. Significance was determined by mixed-effects REML regression with a Kenward-Rogers degrees of freedom approximation. (D) Representative track plots of blebbistatin and DMSO-treated cells migrating in 2D on 10 µg/ml fibronectin. n.s. = not significant; *, p < 0.05; **, p < 0.01.