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. 2021 Aug 15;32(17):1545–1556. doi: 10.1091/mbc.E21-02-0060

Movie S2.

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Directed cell migration of SAS6 KO PLB-985 cells, corresponds to Figure 4. Luciferase CRISPR control and SAS6 KO PLB-985 cells +/- centrinone migrating on 10 μg/mL fibronectin towards an fMLP gradient (0.5 μM) in a microfluidic device. The frame rate is 10 frames per second. Cells were labeled with Calcein AM. Images were acquired every 30 seconds for 45 minutes.