Antibodies, Reagent, or Chemical	Source	Identifier
Antibodies			Dilutions
Mouse anti-human α-Tubulin	Cell Signaling	3873S	1:500 (IF)
Rabbit anti-human β-Actin	Sigma-Aldrich	SAB5500001	1:1000 (WB)
Mouse anti-human Centrin	EMD-Millipore	04-1624	1:200 (IF)
Rabbit anti-human Giantin	Atlas Antibodies	HPA011008	1:250 (IF)
Mouse anti-human GM130	BD Transduction Laboratories	610823	1:250 (IF), 1:500 (WB)
Rabbit anti-human Pericentrin	Abcam	4448	1:1000 (IF)
Mouse anti-human SAS6	Santa Cruz Biotechnology	sc-81431	1:500 (WB)
Mouse anti-human AKAP450	BD Transduction Laboratories	611518	1:250 (IF)
Goat anti-mouse IgG2a Alexa Fluor 488	Invitrogen	A-21131	1 µg/ml (IF)
Goat anti-mouse Alexa Fluor 488	Invitrogen	A-11001	1 µg/ml (IF)
Goat anti-mouse Alexa Fluor 555	Invitrogen	A32727	1 µg/ml (IF)
Goat anti-mouse Alexa Fluor 680	Invitrogen	A-21057	1:10,000 (WB)
Goat anti-mouse Alexa Fluor 790	Invitrogen	A11357	1:10,000 (WB)
Goat anti-mouse IgG (H&L) Antibody Dylight 800 Conjugated	Rockland Immunochemicals	610-140-002-0.5	1:10,000 (WB)
Goat anti-rabbit IgG Alexa Fluor 568	Invitrogen	A-11011	1 µg/ml (IF)
Goat anti-rabbit IgG Alexa Fluor 647	Invitrogen	A-21244	1 µg/ml (IF)
Goat anti-rabbit Alexa Fluor 680	Invitrogen	A-21076	1:10,000 (WB)
Goat anti-rabbit Alexa Fluor 790	Invitrogen	A11369	1:10,000 (WB)
Goat ant-Rabbit IgG (H&L) Antibody Dylight 800 Conjugated	Rockland Immunochemicals	611-145-002-0.5	1:10,000 (WB)
Reagents and Chemicals
Bovine Serum Albumin	Sigma-Aldrich	A7906
D-PBS	Corning	21-031-CV
HEPES	Corning	25060CI
Human Serum Albumin	Lee BioSolutions	101-15-100
Nocodazole	Sigma-Aldrich	M1404	10 µM
Taxol	Tocris Bioscience	10-917-0	10 µM
Y-27632 (ROCK inhibitor)	Tocris Bioscience	1254	10 µM