Mouse anti-human α-Tubulin |
Cell Signaling |
3873S |
1:500 (IF) |
Rabbit anti-human β-Actin |
Sigma-Aldrich |
SAB5500001 |
1:1000 (WB) |
Mouse anti-human Centrin |
EMD-Millipore |
04-1624 |
1:200 (IF) |
Rabbit anti-human Giantin |
Atlas Antibodies |
HPA011008 |
1:250 (IF) |
Mouse anti-human GM130 |
BD Transduction Laboratories |
610823 |
1:250 (IF), 1:500 (WB) |
Rabbit anti-human Pericentrin |
Abcam |
4448 |
1:1000 (IF) |
Mouse anti-human SAS6 |
Santa Cruz Biotechnology |
sc-81431 |
1:500 (WB) |
Mouse anti-human AKAP450 |
BD Transduction Laboratories |
611518 |
1:250 (IF) |
Goat anti-mouse IgG2a Alexa Fluor 488 |
Invitrogen |
A-21131 |
1 µg/ml (IF) |
Goat anti-mouse Alexa Fluor 488 |
Invitrogen |
A-11001 |
1 µg/ml (IF) |
Goat anti-mouse Alexa Fluor 555 |
Invitrogen |
A32727 |
1 µg/ml (IF) |
Goat anti-mouse Alexa Fluor 680 |
Invitrogen |
A-21057 |
1:10,000 (WB) |
Goat anti-mouse Alexa Fluor 790 |
Invitrogen |
A11357 |
1:10,000 (WB) |
Goat anti-mouse IgG (H&L) Antibody Dylight 800 Conjugated |
Rockland Immunochemicals |
610-140-002-0.5 |
1:10,000 (WB) |
Goat anti-rabbit IgG Alexa Fluor 568 |
Invitrogen |
A-11011 |
1 µg/ml (IF) |
Goat anti-rabbit IgG Alexa Fluor 647 |
Invitrogen |
A-21244 |
1 µg/ml (IF) |
Goat anti-rabbit Alexa Fluor 680 |
Invitrogen |
A-21076 |
1:10,000 (WB) |
Goat anti-rabbit Alexa Fluor 790 |
Invitrogen |
A11369 |
1:10,000 (WB) |
Goat ant-Rabbit IgG (H&L) Antibody Dylight 800 Conjugated |
Rockland Immunochemicals |
611-145-002-0.5 |
1:10,000 (WB) |
Reagents and Chemicals |
|
Bovine Serum Albumin |
Sigma-Aldrich |
A7906 |
|
D-PBS |
Corning |
21-031-CV |
|
HEPES |
Corning |
25060CI |
|
Human Serum Albumin |
Lee BioSolutions |
101-15-100 |
|
Nocodazole |
Sigma-Aldrich |
M1404 |
10 µM |
Taxol |
Tocris Bioscience |
10-917-0 |
10 µM |
Y-27632 (ROCK inhibitor) |
Tocris Bioscience |
1254 |
10 µM |