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Faa4-mEos2 single molecule signal (left) under 561 nm excitation from cells grown to stationary phase. Nile red signal (right) under 488 nm excitation visualizes a fraction of LDs moving randomly inside vacuole and the other fraction immobile. Faa4-mEos2 signal is also visible from LDs moving randomly inside (top cell). Scale bar: 1 μm.
