FIGURE 7:

B4GalT1 is efficiently relocalized to an endosomal compartment in response to CQ treatment. (A) HeLa cells expressing endogenously tagged B4GalT1-GFP were left untreated or treated for 3 h with 0.1 mM CQ and stained for giantin and GPP130. (B) RPE1 WT cells were left untreated or treated for 3 h with 0.1 mM CQ and stained for giantin, GPP130, and B4GalT1. Arrows in the merged image indicate putative endocytic vesicles. (C) Quantification of colocalization of GPP130 (left panel) and B4GalT1-GFP (right panel) with giantin in 40 cells using Pearson’s correlation coefficient (HeLa cells). (D) Quantification of colocalization of GPP130 (left panel) and B4GalT1 (right panel) with giantin in 40 cells using Pearson’s correlation coefficient (RPE1 cells). Statistical analysis was done using GraphPad Prism (paired t test). Each dot on the bar graph indicates the colocalization in cells per field. ****P ≤ 0.0001, ***P ≤ 0.001, *P ≤ 0.05.