Movie S1.

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Two-color super-resolution Airyscan live cell imaging of cells expressing B4GalT1-GFP and ST-RFP. HeLa cells were CRISPR knock-in with GFP to express endogenous B4GalT1-GFP and were transiently transfected with plasmid expressing ST-RFP. Live cell imaging of cells was done for 7 minutes with time interval of 3 seconds. Time lapse corresponds to Figure 8. Note the appearance of both enzymes in the Golgi membranes, rapidly moving vesicles and endosome-like tubular compartment. The time stamp represents seconds.