TABLE 1:

List of antibodies used.

Antibody	Source/Catalog #	Species	WB dilution	IF dilution
COG3	Lupashin lab	Mouse	1:1000	1:1000
COG8	Sigma SAB4200427	Rabbit	1:1000	1:500
Giantin	Covance PRB-114C	Rabbit	–	1:1000
GM130	BD Biosciences, 610823	Mouse	–	1:500
GM130	CalBiochem, CB1008	Rabbit	–	1:300
β-actin	Sigma, A5441	Mouse	1:1000	–
TGN46	Bio-Rad, AHP500G	Sheep	1:2000	–
CD63	DSHB, H5C6-C	Mouse	–	1:200
PDI	Affinity Bio Reagents, MA3-019	Rabbit	–	1:1000
LAMP2	DSHB, H4B4	Mouse	1:500	–
Cathepsin D	Sigma, C0715	Mouse	1:500	–
TMEM165	Sigma, HPA038299	Rabbit	1:500	–
MGAT1	Abcam, ab180578	Rabbit	1:500	–
B4GalT1	R&D Systems, AF-3609	Goat	1:500	1:300
ST6Gal1	R&D Systems, AF-5924	Goat	1:500	–
GPP130	Covance, PRB-144C	Rabbit	1:1000	–
VPS53	Thermo Fisher, PA520548	Rabbit	1:1000	–
VPS54	St John’s lab, STJ115181	Rabbit	1:1000	–
GALNT2 (GalNacT2)	Thermo Fisher, PA521541	Rabbit	1:1000	–
IRDye 680 anti-Mouse	LiCOR/926-68170	Goat	1:40000	–
IRDye 800 anti-Rabbit	LiCOR/926-32211	Goat	1:40000	–
IRDye 800 anti-Goat	LiCOR/926-32214	Donkey	1:40000	–
Alexa Fluor 647 anti-Rabbit	Jackson Immuno Research/711-605-152	Donkey	1:500	1:1000
Alexa Fluor 647 anti-Mouse	Jackson Immuno Research/715-605-151	Donkey	1:500	1:1000
Alexa Fluor 647 anti-Goat	Jackson Immuno Research/ 705-605-147	Donkey	–	1:1000
DyLight 647 anti-Sheep	Jackson Immuno Research/713-605-147	Donkey	–	1:1000
anti-Rabbit Cy3	Jackson Immuno Research/711-165-152	Donkey	–	1:1000
anti-Mouse Cy3	Jackson Immuno Research/715-165-151	Donkey	1:500	1:1000
Alexa Fluor 488 anti-Rabbit	Jackson Immuno Research/711-545-152	Donkey	1:500	1:1000
Alexa Fluor 488 anti-Mouse	Jackson Immuno Research/715-545-151	Donkey	1:500	1:1000