Figure 6. Schematic pipeline of Llamanade.
The software is composed of five main modules: 1) structural modeling (purple), 2) sequence annotation (yellow), 3) sequence analysis (green), 4) structural analysis (pink), and 5) humanization score (brown). The input is Nb sequence information in a fasta format. Provided availability, additional structural input can be uploaded to facilitate the analysis. Nb sequences and structures will be first annotated by Llamanade to define CDRs and FRs. The sequence analysis module then selects candidate residues for humanization by comparing the sequence to the VHhuman residue frequency matrix. The structural analysis including degree of burial, intra- and intermolecular interactions will be performed to evaluate the feasibility of humanization for candidate residues. The outputs are the humanized Nb sequence, structure and humanization score.