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. Author manuscript; available in PMC: 2022 May 14.
Published in final edited form as: Circ Res. 2021 May 13;128(10):1451–1467. doi: 10.1161/CIRCRESAHA.121.318159

Figure 4. Stretch-induced titin phosporylation.

Figure 4.

In a Langendorff preparation an acute volume load causes a brisk rise in LV end-diastolic pressure (LVEDP), which gradually falls over a 15 minute period. Subsequent removal of the volume load causes LVEDP to drop below baseline without full recovery over the next 10 minutes period (Left hand panel; *,†,‡: p<0.001). In stretched muscle strips, titin phosphorylation (total and isoform specific) was signficantly higher than in non-stretched muscle strips (Middle panel) as also evident from Pro-Q diamond staining (Right hand panel). Stretch induced effects disappeared following inhibition of PKG (PKGi) and were absent in hypertrophied myocardium.