Figure 4:

Bridge linker preparation described in Support Protocol 1. A gel picture of electrophoresis for the bridge linker DNA oligos. Sample Lanes 2 and 8 (by the ladders) are for the single strand top and bottom oligo, respectively. Lanes 3–7 are for the annealed bridge linkers under five different annealing ratio of top over bottom oligos, 1:1, 1.5:1, 2:1, 1:1.5, and 1:2, respectively. Lane 1 and 9 show the molecular markers of a 25 bp ladder. As shown in this example, the ratio of top oligo:bottom oligo at 1.5:1 has the best result to get the maximum double stranded linkers. All other ratios showed remaining single strand oligos that were not annealed into double stranded linkers.