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. 2021 Jul 28;17(7):e1009683. doi: 10.1371/journal.pgen.1009683

Fig 1. Identification of Alan Shepard (Shep) as an RNA binding protein of DAPALR.

Fig 1

(A) Genomic organization of Dsx1 gene in Daphnia magna. Exons are indicated by boxes; red: Dsx1 β, blue: Dsx1 α. The ORF is indicated by a black box. The position and orientation of DAPALR are indicated by the green box and arrow. (B) Outline of the RNA pulldown assay for the identification of the RNA binding proteins. (C) Domain structure of Shep in D. magna and its similarity with its orthologs in other species. The blue boxes represent the Shep ORF. The two RRMs (RNA Recognition Motifs) domains are shown in green boxes within the ORF. (D) Temporal expression profile of Shep in embryonic developmental stages. Results are shown as relative expression normalized with the ribosomal protein L32. Error bars indicate the standard error of the mean (n = 3), not significant in all points (Student’s T-test between male and female).