Evaluation of CT contrast-induced nephrotoxicity. 30 week old male and female C57BL/6 mice were injected either 150 μL of the diluted contrast agent Iopamidol (1:1 dilution with ultra-pure normal saline), or ultra-pure saline solution intravenously only, via lateral tail injection to perform the CT scan. Urine and blood were collected at baseline and 1 week after treatment to measure (A) serum BUN, (B) serum creatine, (C) body weight, (D) urine protein mg/dL, (E) urine creatinine (mg/dL) (F) urine protein to creatinine ration = 5 in each group; groups were compared by two-way ANOVA using graph prism pad software.