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. 2020 Dec 15;2(5):690–706. doi: 10.1016/j.jaccao.2020.11.004

Figure 5.

Figure 5

Effect of Targeted Gene Inhibition on DOX-Induced Cardiotoxicity in hiPSC-CMs

(A) RT-qPCR analysis of PGP17 hiPSC-CMs (3 biological replicates, each containing 3 technical replicates) treated with 0.1 μmol/L DOX demonstrated a significant increase in gene expression levels of ZNF827, ELAC2, and PI3KR2.(B) PGP17 and (C) PGP14 hiPSC-CMs were pre-treated with DMSO or an inhibitor for 24 h and then exposed to DOX for 24 h. Dose-response curves for cell index, that is, CM viability, and IC50 values for DOX with and without inhibitors are shown. Data are mean ± SD for 3 independent replicates. ∗p < 0.05; ∗∗p < 0.01. PGP = Personal Genome Project; RT-qPCR = quantitative reverse transcription polymerase chain reaction; other abbreviations as in Figure 4.