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. 2021 Jul 26;8:686086. doi: 10.3389/fmolb.2021.686086

FIGURE 2.

FIGURE 2

Biophysical studies to elucidate the effect of pT3, OxM8, and AcK6 on mutant Httex1 aggregation. (A) Httex1 aggregation studies (at 5 µM) of Httex1-43Q-oxM8, Httex1-43Q-oxM8/pT3, Httex1-43Q-oM8/AcK6, and unmodified mHttex1 monitored by UPLC sedimentation assay to determine the proportion of remaining monomer (error bars, S.D. derived from n = 3 independent experiments fitted with exponential decay). (B) Secondary structure analysis of unmodified Httex1-43Q and Httex1-43Q-oxM8 by CD at 0 and 48 h of aggregation. (C) Electron microscopy after 48 h of aggregation (scale bars = 200 nm). (D) Secondary structure analysis of Httex1-43Q-oxM8 and Httex1-43Q-oxM8 by CD at 0 and 48 h post-aggregation initiation.