Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | BV421 anti-human IgG (monoclonal) | Biolegend | RRID:AB_2562176; Cat# 409318 | FACS (1:200) |
| Antibody | APC anti-human IgM (monoclonal) | Biolegend | RRID:AB_493011; Cat# 314510 | FACS (1:200) |
| Antibody | PE anti-human IgA (monoclonal) | Miltenyi Biotech | RRID:AB_2733860; Cat# 130-114-002 | FACS (1:200) |
| Antibody | Anti-SARS-CoV-2 S2 clone D001 (monoclonal) | SinoBiological | RRID:AB_2857932; Cat# 40590-D001 | FACS |
| Antibody | Alexa488 anti-SARS-CoV-2 nucleoprotein (monoclonal) | Produced in-house | CR3009 | IF |
| Recombinant DNA reagent | pcDNA3-SARS-CoV-2_WT spike | Dr Massimo Pizzato, University of Trento, Italy | Wuhan spike sequence | Transfected construct |
| Recombinant DNA reagent | pcDNA3-SARS-CoV-2_D614G spike | Dr Massimo Pizzato, University of Trento, Italy | Wuhan spike sequence with D614G mutation and cytoplasmic tail deletion | Transfected construct |
| Recombinant DNA reagent | pcDNA3-SARS-CoV-2_B.1.1.7 spike | This paper | B.1.1.7 spike sequence | Transfected construct |
| Recombinant DNA reagent | pcDNA3-SARS-CoV-2_ B.1.351 spike | This paper | B.1.351 spike sequence | Transfected construct |
| Cell line (Homo sapiens) | HEK293T | Cell Services facility at the Francis Crick Institute | RRID:CVCL_0063; CVCL_0063 | |
| Cell line (Chlorocebus sp.) |
Vero E6 | Dr Björn Meyer, Institut Pasteur, Paris, France | CRL-1586 | |
| Cell line (Chlorocebus sp.) |
Vero V1 | Prof. Steve Goodbourn, St. George’s, University of London, London, UK | CCL-81 | |
| Other | SARS-CoV-2 | hCoV-19/England/02/2020 | Respiratory Virus Unit, Public Health England, UK | Wuhan strain |
| Other | SARS-CoV-2 | hCoV-19/England/204690005/2020 | Public Health England (PHE), UK, through Prof. Wendy Barclay, Imperial College London, London, UK | B.1.1.7 strain |
| Other | SARS-CoV-2 | 501Y.V2.HV001 Cele et al., 2021 |
B.1.351 strain |