Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (S. cerevisiae) | Full list of yeast strains is presented in Supplementary file 1. | |||
| Recombinant DNA reagent | pBS-SK-Halo-NatMX (plasmid) | Ranjan et al., 2020 | ||
| Recombinant DNA reagent | pUG72 (plasmid) | Euroscarf, Germany | pUG72 (P30117)-Euroscarf | |
| Sequence-based reagent | Full list of oligonucleotides is presented in Supplementary file 2. | |||
| Chemical compound, drug | JF552-HaloTag ligand | Zheng et al., 2019 | N/A | |
| Chemical compound, drug | JF646-HaloTag ligand | Grimm et al., 2015 | N/A | |
| Chemical compound, drug | #1.5 Micro Coverglass – 25 mm Diameter | Electron Microscopy Sciences | 72225–01 | |
| Software, algorithm | ImageJ (1.52 p) | ImageJ | RRID:SCR_003070
https://imagej.net/ |
|
| Software, algorithm | Diatrack 3.05 | Vallotton and Olivier, 2013 | http://www.diatrack.org/index.html | |
| Software, algorithm | GraphPad Prism version 8.4.2 | GraphPad Software, Inc. | RRID:SCR_002798
http://www.graphpad.com |
|
| Software, algorithm | Sojourner package | Carl Wu lab | https://rdrr.io/github/sheng-liu/sojourner/ | |
| Software, algorithm | Spot-On | Hansen et al., 2018 | https://spoton.berkeley.edu/ | |
| Software, algorithm | vbSPT | Persson et al., 2013 | http://vbspt.sourceforge.net/ | |
| Software, algorithm | Radius of confinement calculation | Lerner et al., 2020 | https://data.mendeley.com/datasets/wctzwpp9h2/2 | |
| Software, algorithm | Custom Matlab script | This paper, Mendeley Data | http://dx.doi.org/10.17632/ydwcx9yhpp.2 |