Figure 3. VASP is required for DdMyo7 cortical recruitment.
(A) Confocal images of wild type, myo7 null, vasp null or dia2 null cells expressing RFP-Lifeact (actin). (B) Violin plot of number of filopodia per cell (see also Figure 3—source data 1). (C) Micrographs of cells expressing GFP-DdMyo7 (top) or GFP-VASP in myo7 null, vasp null or dDia2 null cells. (D) Quantification of the cortical band (0.8 µm of periphery) relative to the cytoplasmic intensity of either GFP-Myo7 or GFP-VASP. (A,C) Scale bar is 10 µm (see also Figure 3—source data 3). (B, D) One-way ANOVA with multiple comparison correction or student’s t-test to compare GFP-VASP, ns, not significant, p***<0.001, p****<0.0001, circles are experimental means (see also Figure 3—source data 2 and 4).
Figure 3—source data 1. Number of filopodia per cell counted for each cell in strains in Figure 3B.
elife-68082-fig3-data1.xlsx (12.9KB, xlsx)
Figure 3—source data 2. Statistical test results for Figure 3B.
elife-68082-fig3-data2.xlsx (10.5KB, xlsx)
Figure 3—source data 3. Cortex: cell ratio values for each cell for lines analyzed in Figure 3D.
elife-68082-fig3-data3.xlsx (22.5KB, xlsx)
Figure 3—source data 4. Statistical test results for Figure 3D.
elife-68082-fig3-data4.xlsx (10.7KB, xlsx)
Figure 3—figure supplement 1. VASP is not present in DdMyo7 immunoprecipitates.
(A) GFP-DdMyo7 or GFP-DdMyo7-KKAA (see Figure 5) were immunoprecipitated from a clarified lysate (post nuclear spin sup; PNS) using GFP nanobody beads. Western blot analysis of PNS and immunoprecipitate pellet (IP Pell) probed with antibodies for DdMyo7, actin, and DdVASP.