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. 2021 May 27;10:e68082. doi: 10.7554/eLife.68082

Figure 5. Reduced cortical recruitment of DdMyo7 by VASP mutants.

Figure 5.

(A) Schematic of domains of DdVASP (top) and proposed interaction of DdVASP wildtype, monomeric (∆tet), and F-actin binding (FAB K-E) mutant with actin filaments. (B) Quantification of the cortical recruitment of GFP-DdMyo7 co-expressed in the vasp null with wildtype or mutant DdVASP (non-fluorescent) rescue constructs (see also Figure 5—source data 1). (C) Quantification of GFP-DdMyo7 positive filopodia per cell of vasp null cells with wildtype or mutant DdVASP rescue constructs (see also Figure 5—source data 3). (B–C) Circles represent experimental means. One-way ANOVA with multiple comparison correction, p****<0.0001, ns not significant (see also Figure 5—source data 2 and 4). (D) Clustal Omega alignment of Dictyostelium and human VASP sequences with conserved domains highlighted and mutated residues starred. (E) Micrographs of GFP-DdMyo7 in vasp nulls, or vasp nulls expressing wildtype DdVASP or mutant DdVASP rescue constructs. Scale bar is 10 µm.

Figure 5—source data 1. Cortex: cell ratio values for each cell for lines analyzed in Figure 5B.
Figure 5—source data 2. Statistical test results for Figure 5B.
Figure 5—source data 3. Filopodia per cell values for each cell for lines analyzed in Figure 5C.
Figure 5—source data 4. Statistical test results for Figure 5C.