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. 2021 May 27;10:e68082. doi: 10.7554/eLife.68082

Figure 6. VASP-mediated actin assembly relieves DdMyo7 head-tail autoinhibition to promote targeting and filopodia formation.

Figure 6.

(A) (top) Diagrams depicting mutants analyzed. (bottom) Micrographs of GFP-DdMyo7 fusion proteins in control and vasp null cells, scale bar is 10 μm. Arrows indicate cortical enrichment of DdMyo7. (B) Quantification of cortical recruitment of GFP-DdMyo7 and variants in vasp null cells (see also Table 2 and Figure 6—source data 1). The line represents the mean GFP-DdMyo7 recruitment in wild type cells. (C) Comparison of cortical targeting of activated DdMyo7-KKAA or tail in vasp null versus control cells (see also Figure 6—source data 1 and 2). (D) Quantification of number of filopodia per cell in control or vasp null cells (see also Table 2; Figure 6—source data 3). (B–D) Circles represent experimental means. One way ANOVA with multiple comparison test, ns not significant, p***<0.001, p****<0.0001, ns, not significant (see also Figure 6—source data 2 and 4). (E) Quantification of the cortical band intensity variation of DdMyo7-KKAA in control versus vasp null cells (see also Figure 6—source data 5). Students t-test ****p<0.0001.

Figure 6—source data 1. Cortex: cell ratio values for each cell for lines analyzed in Figure 6B.
Figure 6—source data 2. Statistical test results for Figure 6B,C.
Figure 6—source data 3. Filopodia per cell values for each cell for lines analyzed in Figure 6D.
Figure 6—source data 4. Statistical test results for Figure 6D.
Figure 6—source data 5. Values of the cortical standard deviation measurements (cortical asymmetry) measured for each cell for Figure 6E.