Table 1. Cortical recruitment ratio of DdMyo7 and filopodia per cell for GFP-DdMyo7/myo7 null cells treated with various pharmacological compounds.
Table 1—source data 1. Filopodia per cell values for each cell for lines analyzed in Table 1.
elife-68082-table1-data1.xlsx (32.2KB, xlsx)
Table 1—source data 2. Statistical test results for filopodia per cell data in Table 1 (see also Figure 2—figure supplement 1).
elife-68082-table1-data2.xlsx (10.5KB, xlsx)
Table 1—source data 3. Cortex: cell ratio values for each cell for lines presented in Table 1.
elife-68082-table1-data3.xlsx (53.4KB, xlsx)
Table 1—source data 4. Statistical test results for cortex: cell ratio data in Table 1 (see also Figure 2—figure supplement 1).
elife-68082-table1-data4.xlsx (9.4KB, xlsx)
| Controls | Buffer only | 1% DMSO | Noco. Ctrl. |
|---|---|---|---|
| Percent of cells with filopodia | 42 | 38 | 29 |
| Filopodia number + SEM | 2.01 ± 0.11 | 1.97 ± 0.09 | 1.5 ± 0.17 |
| Cortex:cytoplasm ratio + SEM | 1.18 ± 0.01 | 1.2 ± 0.02 | 1.31 ± 0.03 |
| N, n | 4, 238 | 3, 229 | 4138 |
| CytochalasinA | 1 µM | 5 µM | 30 µM |
| Percent of cells with filopodia | 0 | 8.5 | 19 |
| Filopodia number + SEM | 0 | 1.3 ± 0.04 | 0.1 ± 0.06 |
| Cortex:cytoplasm ratio + SEM | 1.02 ± 0.02 | 1.07 ± 0.01 | 1.04 ± 0.02 |
| N, n | 3, 66 | 3, 234 | 1, 42 |
| LatrunculinA | 1 µM | 5 µM | 15 µM |
| Percent of cells with filopodia | 19 | 4.7 | 20 |
| Filopodia number + SEM | 1.73 ± 0.06 | 1.33 ± 0.05 | 0.24 ± 0.06 |
| cortex:cytoplasm ratio + SEM | 1.12 ± 0.01 | 1.02 ± 0.01 | 1.08 ± 0.02 |
| N, n | 4, 387 | 3, 127 | 1, 74 |
| Jasplakinolide | 15 nM | 50 nM | 100 nM |
| Percent of cells with filopodia | 55 | 72 | 48 |
| Filopodia number + SEM | 1.47 ± 0.23 | 3.89 ± 0.2 | 2.21 ± 0.09 |
| Cortex:cytoplasm ratio + SEM | 1.19 ± 0.03 | 1.34 ± 0.03 | 1.24 ± 0.02 |
| N, n | 1, 84 | 3, 219 | 3, 337 |
| LY294002/wortmannin | 20 μM LY294002 | 60 μM LY294002 | 2 μM WM |
| Percent of cells with filopodia | 22 | 40 | 30 |
| Filopodia number + SEM | 0.56 ± 0.44 | 0.87 ± 0.12 | 0.84 ± 0.16 |
| Cortex:cytoplasm ratio + SEM | 1.22 ± 0.08 | 1.17 ± 0.04 | 1.16 ± 0.02 |
| N, n | 2, 9 | 4, 194 | 2, 101 |
| CK666 | 25 μM | ||
| Percent of cells with filopodia | 10.7 | ||
| Filopodia number + SEM | 0.145 ± 0.03 | ||
| Cortex:cytoplasm ratio + SEM | 1.12 ± 0.01 | ||
| N, n | 3, 366 | ||
| Nocodazole | 5 μM | 15 μM | 50 μM |
| Percent of cells with filopodia | 32 | 14 | 8 |
| Filopodia number + SEM | 1.74 ± 0.22 | 1.27 ± 0.11 | 1.1 ± 0.09 |
| Cortex:cytoplasm ratio + SEM | 1.15 ± 0.03 | 1.12 ± 0.01 | 1.1 ± 0.01 |
| N, n | 2, 59 | 4, 133 | 2, 127 |
Percent of all cells with at least one filopodia. Average number of filopodia per cells from cells with at least one filopodia. Cortex:cytoplasm ratio is intensity ratio of a 0.8 µm band around the periphery compared to the cytoplasm. N is number of experiments, n is number of cells. SEM is standard error of the mean. See also Table 1—source data 1–4.