. 2021 May 27;10:e68082. doi: 10.7554/eLife.68082

Table 2. Quantification of filopodia number and cortical targeting.

myo7-

DdMyo7

KKAA

E386V

E386V-KKAA

I426A

CAAX

Tail

GFP-VASP

percent of cells with filopodia

n.c.

filopodia number + SEM

2.53 ± 0.15

5.32 ± 0.33

1 ± 0

1.5 ± 0.09

1.75 ± 0.15

2.87 ± 0.14

n.c.

cortex:cytoplasm ratio + SEM

1.18 ± 0.02

1.67 ± 0.06

1 ± 0.01

1.32 ± 0.04

1.19 ± 0.04

1.39 ± 0.03

1.61 ± 0.07

1.18 ± 0.29

N, n

4, 198

3, 133

3, 186

3, 59

3, 37

3, 237

3, 55

3, 91

vasp-

GFP-DdMyo7

VASP-∆TET

VASP -WT

VASP-FAB-K-E

GFP-VASP

DdMyo7-CAAX

DdMyo7-Tail

DdMyo7-KKAA

GFP-Dia2

mCherry-Dia2-CA

GFP-V1

percent of cells with filopodia

n.c.

filopodia number + SEM

1.25 ± 0.07

3.78 ± 0.31

3.07 ± 0.25

1.39 ± 0.03

n.c.

1.2 ± 0.03

n.c.

1 ± 0

0 ± 0

2.03 ± 0.24

0 ± 0

cortex:cytoplasm ratio + SEM

1.06 ± 0.01

1.2 ± 0.02

1.47 ± 0.05

1.19 ± 0.01

1.18 ± 0.33

1.24 ± 0.01

1.35 ± 0.02

1.29 ± 0.02

1.65 ± 0.03

1.82 ± 0.09

1.12 ± 0.02

N, n

3, 118

3, 131

3, 81

3, 322

3, 200

4, 239

3, 213

3, 193

5447

8,94

3,35

Ax2 control

GFP-DdMyo7

GFP-DdMyo7-CAAX

GFP-DdMyo7-Tail

GFP-DdMyo7-KKAA

GFP-VASP

percent of cells with filopodia

n.c.

filopodia num ber + SEM

2.36 ± 0.2

2.9 ± 0.15

n.c.

3.65 ± 0.2

n.c.

cortex:cytoplasm ratio + SEM

1.35 ± 0.03

1.45 ± 0.04

1.41 ± 0.02

1.79 ± 0.07

1.16 ± 0.25

N, n

4, 124

5, 266

4, 351

3, 219

4, 216

The percent of all cells with at least one filopodia. Average number of filopodia per cells from cells with at least one filopodia. Cortex:cytoplasm ratio is intensity ratio of a 0.8 µm band around the periphery compared to the cytoplasm. N is number of experiments, n is number of cells. SEM is standard error of the mean. GFP-VASP and GFP-DdMyo7-Tail fail to efficiently target to filopodia tips and thus were not counted (n.c.) in this analysis. (See source data: Figure 4—source data 1 and 2; Figure 5—source data 1–3; Figure 6—source data 1–5; Figure 7—source data 1, 2, 4 and 5).