Figure 8. Loss of Gal9 in NZB/W mice leads to accelerated autoimmunity.

(A) Titers of dsDNA-specific IgG in sera of NZB/W mice (as indicated) monitored over time. (B) Protein secretion in the urine of NZB/W mice monitored over time. (C) Representative plots and (D) summary proportion of splenic GC B cells in NZB/W mice (as indicated). (E) Representative plots and (F) summary proportion of antibody secreting cells (ASC) in the spleen of NZB/W mice. (G) Proportion of long-lived ASC lacking B220 expression. (H) Circulating IgG-containing immune complex (ICs) titers. (I) Proportion of CD5+ B-1a cells in the peritoneal cavity of NZB/W mice. (J) Proportion of CD19+ CD43+ B-1 cells in the spleen of NZB/W mice. (K) Proportion of splenic CD5+ B-1a cells. (L) Circulating phosphoryl-choline specific IgM titers in NZB/W mice. Data in (A) and (B) are representative of at least 14 biological replicates, data in (C–L) are representative of eight biological replicates. Data represent mean and SEM. Statistical significance was assessed by Mann–Whitney, *p≤0.05, **p≤0.01, ***p≤0.001.