| Strain, strain background (Mus musculus) |
C57BL/6 |
Charles River Laboratories |
Strain code: 027 |
|
| Strain, strain background (Mus musculus) |
Gal9-/- |
Steven Beverley (Washington University) on behalf of the Scripps Research Institute |
Gal9KO |
|
| Strain, strain background (Mus musculus) |
Nr4a1-eGFP (Nur77-GFP) |
Jackson Laboratories |
Strain code: 016617 |
|
| Strain, strain background (Mus musculus) |
New Zealand Black (NZB/BINJ) |
Jackson Laboratories |
Strain code: 000684 |
|
| Strain, strain background (Mus musculus) |
New Zealand White (NZW/LacJ) |
Jackson Laboratories |
Strain code: 001058 |
|
| Strain, strain background (Mus musculus) |
MD4 |
Jackson Laboratories |
Strain code: 002595 |
|
| Strain, strain background (Mus musculus) |
µMT |
Jackson Laboratories |
Strain code: 002288 |
|
| Strain, strain background (Mus musculus) |
MD4/ML5 |
Joan Wither (University of Toronto) |
|
|
| Cell Line (Homo sapiens) |
Male:HEp2 |
ATCC |
Cat#: CCL-23 |
|
| Cell Line (Mus musculus) |
OP9-R7FS |
Juan Carlos Zúñiga-Pflücker (University of Toronto) |
|
|
| Antibody |
Phospho-Tyrosine (p-Tyr) |
Sunnybrook Research Institute |
|
Clone:4G10 |
| Antibody |
Anti-IgM F(ab’)2
|
Jackson Immunoresearch |
Cat#: 155-006-020 |
Polyclonal |
| Antibody |
Anti-B220-Pacific Blue |
BioLegend |
Cat#: 103227 |
Clone: RA3-6B2 |
| Antibody |
Anti-CD11b-Pacific Blue |
BioLegend |
Cat#: 101224 |
Clone: M1/70 |
| Antibody |
Anti-Ly6G-FITC |
BioLegend |
Cat#:127606 |
Clone: 1A8 |
| Antibody |
Anti-CD45- Brilliant Violet 510 |
BioLegend |
Cat#: 103138 |
Clone: 30-F11 |
| Antibody |
Anti-CD169-PE-Cyanine7 |
BioLegend |
Cat#: 142412 |
Clone: 3D6.122 |
| Antibody |
Anti-F4/80-Alexa Fluor 700 |
BioLegend |
Cat#: 123129 |
Clone: BM8 |
| Antibody |
Anti-CD11c-APC |
BioLegend |
Cat#: 117310 |
Clone: N418 |
| Antibody |
Anti-CD64-PE |
BioLegend |
Cat#: 139303 |
Clone: X54-5/7.1 |
| Antibody |
Anti-MHCII (I-A/I-E)-Brilliant Violet 605 |
BioLegend |
Cat#: 107639 |
Clone: M5/114.15.2 |
| Antibody |
Anti-CD8-APC |
BioLegend |
Cat#: 100712 |
Clone: 53-6.7 |
| Antibody |
Anti-CD4-Brilliant Violet 510 |
BioLegend |
Cat#: 100449 |
Clone: GK1.5 |
| Antibody |
Anti-CD23-APC |
BioLegend |
Cat#: 101620 |
Clone: B3B4 |
| Antibody |
Anti-CD21/35-APC-Cyanine7 |
BioLegend |
Cat#: 123418 |
Clone: 7E9 |
| Antibody |
Anti-Galectin-9-PE |
BioLegend |
Cat#: 137904 |
Clone: 108A2 |
| Antibody |
Anti-Galectin-9-Alexa Fluor 488 |
BioLegend |
Cat#: 137908 |
Clone: 108A2 |
| Antibody |
Anti-Galectin-9 |
BioLegend |
Cat#: 137902 |
Clone: 108A2 |
| Antibody |
Anti-CD86-FITC |
BioLegend |
Cat#: 105006 |
Clone: GL-1 |
| Antibody |
Anti-CD86-PE-Cyanine7 |
BioLegend |
Cat#: 105014 |
Clone: GL-1 |
| Antibody |
Anti-IgM-Alexa Fluor 488 |
Jackson Immunoreasearch |
Cat#: 715-547-020 |
|
| Antibody |
Anti-GL7-APC |
BioLegend |
Cat#: 144618 |
Clone: GL7 |
| Antibody |
Anti-CD95-FITC |
BioLegend |
Cat#: 152606 |
Clone: SA367H8 |
| Antibody |
Anti-IgD-Brilliant Violet 605 |
BioLegend |
Cat#: 405727 |
Clone: 11-26c.2a |
| Antibody |
Anti-CD138-Brilliant Violet 605 |
BioLegend |
Cat#: 142516 |
Clone: 281-2 |
| Antibody |
Anti-CD38-PE-Cyanine7 |
BioLegend |
Cat#: 102718 |
Clone: 90 |
| Antibody |
Anti-IgM-Alexa Fluor 647 |
Jackson Immunoresearch |
Cat#: 155-607-020 |
|
| Antibody |
Anti-IgG (FC)-Alexa Fluor 488 |
Jackson Immunoresearch |
Cat#: 155-545-008 |
|
| Antibody |
Anti-PD-1-PE-Cyanine7 |
BioLegend |
Cat#: 109110 |
Clone: RMP1-30 |
| Antibody |
Anti-CXCRV-Brilliant Violet 421 |
BioLegend |
Cat#: 145512 |
Clone: L138D7 |
| Antibody |
Anti-FoxP3-PE |
BioLegend |
Cat#: 126404 |
Clone: MF-14 |
| Antibody |
Anti-IgMa-PE |
BioLegend |
Cat#: 408608 |
Clone: MA-69 |
| Antibody |
Anti-B220-Alexa Fluor 647 |
BioLegend |
Cat#: 103226 |
Clone: RA3-6B2 |
| Antibody |
Anti-CD5-PE-Cyanine 5 |
BioLegend |
Cat#: 100610 |
Clone: 53-7.3 |
| Antibody |
Anti-CD19-Pacific Blue |
BioLegend |
Cat#: 115523 |
Clone: 6D5 |
| Antibody |
Anti-CD43-APC |
BioLegend |
Cat#: 143208 |
Clone: S11 |
| Antibody |
Anti-CD23-PE |
BioLegend |
Cat#: 101607 |
Clone: B3B4 |
| Antibody |
Anti-CD69-Brilliant Violet 510 |
BioLegend |
Cat#: 104531 |
Clone: H1.2F3 |
| Antibody |
Anti-CD5-Alexa Fluor 647 |
BioLegend |
Cat#: 100614 |
Clone: 53-7.3 |
| Antibody |
Anti-SiglecG-APC |
Fisher Scientific |
Cat#: 501123146 |
Clone: SH2.1 |
| Antibody |
Anti-CD22-Alexa Fluor 647 |
BioLegend |
Cat#: 126108 |
Clone: OX-97 |
| Antibody |
Anti-CD180 |
BioLegend |
Cat#:117710 |
Clone:RP/14 |
| Antibody |
Anti-TLR4-Alexa Fluor 488 |
Fisher Scientific |
Cat#: 5016829 |
Clone: UT41 |
| Antibody |
Anti-C3 |
BioLegend |
Cat#: 518106 |
Clone: K13/16 |
| Recombinant Proteins |
Recombinant Galectin-9 |
R&D Systems |
Cat#: 3535-GA-050 |
|
| Chemical compound/drug |
Calf Thymus DNA |
ThermoFisher Scientific |
Cat#: 15633019 |
|
| Chemical compound/drug |
Phosphorylcholine |
Sigma Aldrich |
Cat#: P0378 |
|
| Chemical compound/drug |
Hen Egg Lysozyme |
Sigma Aldrich |
Cat#: 10837059001 |
|
| Chemical compound/drug |
β-lactose |
Sigma Aldrich |
Cat#: L3750 |
|
| Chemical compound/drug |
Streptavidin-Alexa Fluor 647 |
Thermo Fisher Scientific |
Cat#: S21374
|
|
| Chemical compound/drug |
CFSE |
Thermo Fisher Scientific |
Cat#: C34554
|
|
| Chemical compound/drug |
Cell tracker eFluor 450 |
Thermo Fisher Scientific |
Cat#: 65-0842-85 |
|
| Chemical compound/drug |
Puromycin Dihydrochloride |
Thermo Fisher Scientific |
Cat#: A1113803 |
|
| Chemical compound/drug |
LPS |
Invivogen |
Cat#: tlrl-eklps |
|
| Chemical compound/drug |
Imiquimod |
Invivogen |
Cat#: tlrl-imq |
|
| Chemical compound/drug |
Zymosan |
Invivogen |
Cat#: tlrl-zyn |
|
| Chemical compound/drug |
CpG-ODN |
Invivogen |
Cat#: tlrl-1585 |
|
| Chemical compound/drug |
M2 Beads |
Sigma Aldrich |
Cat#: M8823 |
|
| Commercial assay/kit |
B cell Isolation kit |
Stemcell Technologies |
Cat#: 19854 |
|
| Commercial assay/kit |
Mouse IgM Quantitation set |
Bethyl Laboratories |
Cat#: E90-101 |
|
| Commercial assay/kit |
Mouse IgG Quantitation set |
Bethyl Laboratories |
Cat#: E90-131 |
|
| Commercial assay/kit |
Clophosome-A and control liposome kit |
FormuMax |
Cat#: F70101C-AC |
|
| Commercial assay/kit |
Fluorescence quantitation beads |
Bangs Labs |
Cat#: 817 |
|
| Commercial assay/kit |
BCA kit |
Sigma Aldrich |
Cat#: A53225
|
|
| Software, algorithm |
Flowjo v10.6 |
Tree Star |
|
|
| Software, algorithm |
ImageJ |
NIH/LOCI |
|
|
| Software, algorithm |
ThunderSTORM |
Github |
zitmen |
|
| Software, algorithm |
Rstudio |
Rstudio PBC |
|
|
| Software, algorithm |
SMoLR |
Github |
ErasmusOIC |
|
| Software, algorithm |
Spatstat |
Github |
spatstat |
|
