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. 2021 Jul 22;10:e65445. doi: 10.7554/eLife.65445

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© 2021, Zeppilli et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 1. — (A) Schematic representation of experimental design. Top: after injection of rAAVretro-CAGH2B-GFP into PCx, single nuclei were dissociated from 1 mouse and sorted using Fluorescence-activated Nuclei Sorting (FANS). The population of nuclei is selected based on GFP and DRAQ5 (far-red fluorescent DNA dye). Sorted nuclei were sequenced using 10X single-nucleus RNA-seq and integrated with the main dataset sn-R1/R2/R3. Bottom: Same as the top figure but injection was performed into AON. (B) Coronal section showing GFP expression in the injection points for PCx (top) and AON (bottom) after injection of rAAVretro-CAG-H2B-GFP. Neurotrace counterstain in blue. Scale 50μm. PCx: Piriform Cortex; AON: Anterior Olfactory Nucleus.