Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | Srsf1NRS/NRS | This paper | MGI: 645208 | n.a. |
| Cell line (M. musculus) | ESCs Srsf1NRS/NRS | This paper | n.a. | n.a. |
| Cell line (M. musculus) | NSCs Srsf1NRS/NRS | This paper | n.a. | n.a. |
| Cell line (M. musculus) | MEFs Srsf1NRS/NRS | This paper | n.a. | n.a. |
| Antibody | Anti-SRSF1 (mouse monoclonal) | Hanamura et al., 1998 | RRID:AB_2533079 | |
| Antibody | Anti-T7 (mouse monoclonal) | Novagen | Cat#: 69522 | RRID:AB_11211744 |
| Antibody | Anti-tubulin (YOL1/34) |
Abcam | Cat#: ab6161 | RRID:AB_305329 |
| Sequence-based reagent | FAM-5′ Ccdc164 oligonucleotide | IDT | Mm.PT.58.30029646 | |
| Sequence-based reagent | FAM-5′ Tekt3 oligonucleotide | IDT | Mm.PT.58.16533004 | |
| Sequence-based reagent | FAM-5′ Wdr78 oligonucleotide | IDT | Mm.PT.56a.42250015 | |
| Sequence-based reagent | FAM-5′ Wdr65 oligonucleotide | IDT | Mm.PT.58.7105374 | |
| Sequence-based reagent | FAM-5′ Dnaaf1 oligonucleotide | IDT | Mm.PT.58.31362949 | |
| Sequence-based reagent | FAM-5′ Actb oligonucleotide | IDT | Mm.PT.39a.22214843.g | |
| Sequence-based reagent | FAM-5′ Rsph3a oligonucleotide | IDT | Mm.PT.58.29968181.g | |
| Peptide, recombinant protein | Cas9 | TriLink BioTechnologies | L7606 | |
| Peptide, recombinant protein | Q5 High-Fidelity DNA polymerase |
New England Biolabs | M0491 | |
| Peptide, recombinant protein | Recombinant Murine EGF |
Peprotech | 315-09 | |
| Peptide, recombinant protein | Recombinant Human FGF-basic |
Peprotech | 100-18B | |
| Peptide, recombinant protein | Laminin | Sigma Aldrich | L2020 | |
| Commercial assay or kit | RNeasy kit | Qiagen | 74106 | |
| Commercial assay or kit | Turbo DNase | Ambion | AM1907 | |
| Commercial assay or kit | SybrGreen RT-QPCR | Roche | 04707516001 | |
| Commercial assay or kit | T7 Quick High Yield RNA Synthesis kit | NEB | E2050S | |
| Chemical compound, drug | Trizol | Life Technologies | 15596026 | |
| Chemical compound, drug | Hoechst 34580 | Sigma Aldrich | 63493 | |
| Chemical compound, drug | Cycloheximide | Sigma Aldrich | C104450 | |
| Chemical compound, drug | PEG1500 | Roche | 10783641001 | |
| Chemical compound, drug | Lipofectamine 3000 | Thermo Fisher Scientific | L3000015 | |
| Chemical compound, drug | MEM non-essential amino acid | Gibco | 11140050 | |
| Chemical compound, drug | Sodium pyruvate | Gibco | 11360070 | |
| Chemical compound, drug | 2-Mercaptoethanol | Gibco | 31350010 | |
| Chemical compound, drug | Accutase | Stemcell | ||
| Chemical compound, drug | BSA (7.5% solution) | Thermo Scientific | 15260037 | |
| Chemical compound, drug | N-2 supplement (100×) | Thermo Scientific | 17502048 | |
| Chemical compound, drug | B-27 Supplement (50×) | Thermo Scientific | 17504044 | |
| Chemical compound, drug | PD0325901 | Stemcell Technologies | 72182 | |
| Chemical compound, drug | CHIR99021 | Miltenyi Biotec | 130-103-926 | |
| Software, algorithm | ImageJ | NIH | https://imagej.nih.gov/ij/ | |
| Software, algorithm | R (v3.5) | N/A | https://www.r-project.org/ | |
| Software, algorithm | STAR | Dobin et al., 2013 | http://code.google.com/p/rna-star/ | RRID:SCR_004463 |
| Software, algorithm | Salmon | Patro et al., 2017 | https://combine-lab.github.io/salmon/ | RRID:SCR_017036 |
| Software, algorithm | SUPPA2 | Trincado et al., 2018 | https://github.com/comprna/SUPPA | |
| Software, algorithm | WebGestalt2019 | Liao et al., 2019 | http://www.webgestalt.org/ | |
| Software, algorithm | MATS | Park et al., 2013 | http://rnaseq-mats.sourceforge.net/ | RRID:SCR_013049 |
| Software, algorithm | DESeq2 | Love et al., 2014 | http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html | RRID:SCR_015687 |
| Other | Polysomal profiling RNA-seq data | This paper | GSE161828 | Deposited data |
| Other | MEFs RNA-seq data | This paper | GSE157269 | Deposited data |
| Other | mTECs proteomics data | This paper | PXD019859, username: reviewer79803@ebi.ac.uk password: 4HAr4wOY | Deposited data |