Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Mouse anti-strep | Thermofisher | NBP243719 | |
| Antibody | Rabbit anti-thiophosphate ester | Abcam | ab133473 | |
| Antibody | Alexa 680 goat anti-mouse | Thermofisher | A28183 | |
| Antibody | Dylight 800 goat anti-rabbit | Rockland Labs | 611-145-002 | |
| Strain, strain background | Escherichia coli (BL21DE3) | Agilent | 200131 | |
| Strain, strain background | Escherichia coli (XL10Gold) | Agilent | 200314 | |
| Chemical compound, drug | Biotinylated poly(L-lysine)-[g]-poly(ethylene-glycol) (PLL-PEG-Biotin) | SuSoS AG | PLL(20)-G[3.5]-PEG(2)/PEG(3.4)-biotin(50%) | |
| Chemical compound, drug | Streptavidin | Thermofisher | 21135 | |
| Chemical compound, drug | Trolox (6-hydroxy-2,5,6,7,8-tetramethylchroman-2-carbonsaure, 97%) | Acros | AC218940050 | |
| Chemical compound, drug | 3,4-Dihydroxybenzoic Acid (protocatechuic acid) | Sigma-Aldrich | 37580 | |
| Chemical compound, drug | Protocatachuate 3,4-Dioxygenase from Pseudomonas sp. | Sigma-Aldrich | P8279 | |
| Chemical compound, drug | κ-caesin from bovine milk | Sigma-Aldrich | C0406 | |
| Chemical compound, drug | Pierce Bovine Serum Albumin, Biotinylated | Thermofisher | 209130 | |
| Chemical compound, drug | Paclitaxel | Sigma-Aldrich | T7402 | |
| Chemical compound, drug | Pluronic F-157 | Sigma-Aldrich | P2443 | |
| Other | Glass cover slides (18x18-1.5) | Fisher | 12-541A | |
| Other | Superfrost Microscope slides | Fisher | 12-550-143 | |
| Chemical compound, drug | Adenosine 5’-triphosphate disodium salt hydrate | Sigma-Aldrich | A2383 | |
| Chemical compound, drug | Guanosine 5’-triphosphate sodium salt hydrate | Sigma-Aldrich | G8877 | |
| Chemical compound, drug | Bovine Serum Albumin | Sigma-Aldrich | A2058 | |
| Chemical compound, drug | Casein | Sigma-Aldrich | C7078 | |
| Chemical compound, drug | Nonidet P 40 substitute (NP-40) | Sigma-Aldrich | 74385 | |
| Chemical compound, drug | PDMS (polydimethylsiloxane, sylgard 184) | Sigma-Aldrich | 761036 | |
| Chemical compound, drug | DNAseI | NEB | M0303L | |
| Chemical compound, drug | Streptactin Superflow resin | Qiagen | 30002 | |
| Chemical compound, drug | Streptactin XT Superflow resin | IBA | 2-4010-025 | |
| Chemical compound, drug | d-Desthiobiotin | Sigma | D1411 | |
| Chemical compound, drug | D-biotin | CHEM-IMPEX | #00033 | |
| Chemical compound, drug | ATPγS | Thermofisher | 11912025 MG | |
| Chemical compound, drug | p-Nitrobenzyl mesylate | Abcam | ab138910 | |
| Other | Phos-tag gels | Wako | 192–18001 | |
| Chemical compound, drug | GMPCPP | Jena Biosciences | NU-405 | |
| Recombinant protein | Calpain | Sigma | C6108 | |
| Recombinant DNA | Mouse DCLK1 | Transomics | #BC133685 | |
| Recombinant DNA | Lambda phosphatase | Addgene | 79748 | |
| Software, algorithm | FIJI | Schindelin et al., 2012 | https://Fiji.sc/ | |
| Software, algorithm | GraphPad Prism | GraphPad | https://www.graphpad.com/scientific-software/prism/ | |
| Software, algorithm | μManager | Edelstein et al., 2010 | https://micro-manager.org/ |