Figure 5.

Screening of cross-reactivity between HLA-A2^S269–277WT and variant epitopes.A, gating strategy of dual-tetramer enrichment with representative fluorescence-activated cell sorting plots of the unenriched, enriched, and flowthrough fractions of tetramer⁺ populations (donor 2), gated on live/CD14⁻/CD19⁻CD3⁺/CD8⁺ T cells. B, dual-TAME-enriched WT HLA-A2^S269–277–WT-tetramer⁺ populations against the different variant–tetramer⁺ populations from three donors as concatenated fluorescence-activated cell sorting plots. HLA, human leukocyte antigen.