Table 1.
Summary of SPR affinity measurements
| Epitope | Sequence | NR1C |
NR2F |
NR1A |
NR1D |
|---|---|---|---|---|---|
| KD (μΜ) 95% CI | |||||
| S269–277 | YLQPRTFLL | 2.71 | 2.75 | 2.36 | 3.72 |
| 2.30–3.18 | 2.21–3.40 | 1.92–2.89 | 3.33–4.15 | ||
| S269–277P272L | YLQLRTFLL | 272.4 | 170.2 | 157.5 | 163.1 |
| 235.5–320.5 | 160.1–181.5 | 140.9–178.0 | 155.4–171.3 | ||
| S269–277R273S | YLQPSTFLL | N.B. | N.B. | N.B. | N.B. |
| S269–277T274I | YLQPRIFLL | 109.3 | 360.3 | ND | ND |
| 104.2–114.7 | 270.6–520.8 | ||||
| PNRL | YLQQRTFLL | 77.6 | 5.87 | ND | ND |
| 74.9–80.6 | 5.17–6.67 | ||||
| BTRL | YLKPRTFML | >100 | >100 | ND | ND |
| SARS | YLKPTTFML | N.B. | N.B. | ND | ND |
| MERS | KQLPLTFLL | N.B. | N.B. | ND | ND |
| PAR1 | TLDPRSFLL | N.B. | N.B. | ND | ND |
TCR binding to immobilized HLA-A2S269–277 and homologous epitopes (sequence differences to the SARS-CoV-2 epitope are highlighted in bold) were measured in two independent experiments with two replicates. Dissociation constant (KD) (in bold) and confidence interval (95% CI) (in italics) were calculated from all data using a single-site-binding model with KD as a shared variable.
Abbreviations: N.B., no binding; ND, not determined.