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. 2021 Jul 30;2021:6832518. doi: 10.1155/2021/6832518

Figure 5.

Figure 5

miR-146b-5p directly targeted CBX6. (a) TargetScan exhibited the predicted binding site between CBX6 and miR-146b-5p. (b) Dual-luciferase reporter assay was performed to measure the relative luciferase activity in Hep3B and SK-HEP1 cells. ∗∗∗P < 0.001 vs. mimic NC. (c) The protein expression of CBX6 in Hep3B and SK-HEP1 cells was measured by western blot. ∗∗∗P < 0.001 vs. mimic NC. (d) qRT-PCR was used to detect the expression of CBX6 in hepatocellular carcinoma (HCC) tumor tissues and adjacent tissues. ∗∗P < 0.01 vs. normal. (e) The expression of CBX6 was negatively correlated with miR-146b-5p in HCC tissues. (f) The expression of CBX6 was positively correlated with MIR100HG in HCC tissues. (g) qRT-PCR was performed to evaluate the expression of CBX6 in LO2, Hep3B, HepG2, SK-HEP1, and Huh7 cells. ∗∗P < 0.01 vs. LO2.