Table 2:
Results of p53 immunostaining and sequencing analysis
| Case ID | p53 STAINING PATTERNa | TP53 MUTATION (ALLELIC FREQUENCY)b | ||
|---|---|---|---|---|
| Brush Cyto | Tissue | Brush Cyto | Tissue | |
| 1 | wt | wt | No Mutation | - |
| 2 | wt | wt | No Mutation | No Mutation |
| wt | wt | No Mutation | - | |
| 3 | wt | wt | - | - |
| 4 | wt | wt | No Mutation | No Mutation |
| wt | wt | No Mutation | No Mutation | |
| 5 | wt | wt | - | - |
| wt | wt | - | - | |
| 6 | wt | wt | - | - |
| 7 | wt | wt | - | - |
| 8 | wt | wt | - | - |
| 9 | Focal MutD | Focal MutD | No Mutation | No Mutation |
| 10 | wt | wt | No Mutation | - |
| 11 | MutD | MutD | p.R175H (0.81) | p.R175H (0.45) |
| 12 | MutN | MutN | c.559+1G>T, p.? (0.11)c | c.559+1G>T, p.? (0.25)e |
| wt | wt | No Mutation | No Mutation | |
| 13 | MutD | MutD | p.D281N (0.32) | p.D281N (0.44) |
| MutD | MutD | p.D281N (0.17) | p.D281N (0.16) | |
| 14 | wt | wt | - | - |
| 15 | MutN | MutN | p.E271* (0.34) | p.E271* (0.56) |
a.
Staining pattern is reported for the lesion, if present; otherwise, refers to background normal epithelial cells. Patterns of p53 immunoreactivity: wt – wildype, MutD – Mutated, diffuse nuclear pattern, MutN – Mutated, null pattern.
b.
Only non-synonymous variants shown.‘ – ’ indicates sequencing was not performed.
c.
This mutation has been reported to affect splicing, resulting in loss of gene expression.