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. 2021 Aug 9;11:16086. doi: 10.1038/s41598-021-95451-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic of the size and density range of lipoproteins and low-density plasma components. The white box indicates the density and size of high density lipoprotein (HDL) particles captured by this HDL isolation method, as well as the particle diameter (nm) and particle density (g/mL) of the other lipoprotein particles—chylomicrons, very low density lipoproteins (VLDL), intermediate density lipoproteins (IDL), low density lipoproteins (LDL), lipoprotein (a) (Lp(a)), and extracellular vesicles (EVs) present in plasma that are simultaneously isolated as part of this method.