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. 2021 Aug 9;12:4794. doi: 10.1038/s41467-021-25033-4

Fig. 3. TRIM28 promotes NLRP3 inflammasome activation.

Fig. 3

a, b ELISA analysis of IL-1β, TNF, and IL-6 in supernatants of mouse PMs from WT or Trim28CKO mice following priming with LPS for 7 h and subsequent stimulation with ATP, Nig, or poly(dA:dT) for 1 h (a two-tailed t test WT vs. Trim28CKO, *p = 0.011241, **p = 0.005688). c Immunoblot analysis of supernatants (SN) and cell lysates (CL) of mouse PMs from WT or Trim28CKO mice, following priming with LPS and subsequent stimulation with ATP (n = 3 independent experiments). d ELISA analysis of IL-1β in supernatants from mouse PMs transfected with control (Ctrl) siRNA or Trim28 siRNA for 48 h, followed by priming with LPS for 7 h and subsequent stimulation with ATP for 1 h (two-tailed t test Ctrl siRNA vs. Trim28 siRNA, **p = 0.005134). e ELISA analysis of serum levels of IL-1β, TNF, and IL-6 of WT or Trim28CKO mice after i.p. LPS injection (PBS, n = 3; LPS, n = 8 per group, two-tailed t test WT vs. Trim28CKO, ***p = 6 × 10−6). All data are represented as mean ± SD in a, b, d, e (*p < 0.05; **p < 0.01; ***p < 0.001). Similar results were obtained from three independent experiments.