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. 2021 Aug 9;12:4794. doi: 10.1038/s41467-021-25033-4

Fig. 4. TRIM28 inhibits NLRP3 protein degradation.

Fig. 4

a Immunoblot analysis of lysates from HEK293T cells transfected with Myc-NLRP3 and increasing amount of Flag-TRIM28 plasmid. b Immunoblot analysis of lysates of mouse PMs from WT or Trim28CKO mice, following stimulation with LPS. c Immunoblot analysis of lysates of mouse PMs transfected with Ctrl siRNA or Trim28 siRNA for 48 h, following stimulation with LPS. d, e Immunoblot analysis of NLRP3 expression in mouse PMs from WT or Trim28CKO mice stimulated with LPS for 4 h and then treated cycloheximide (CHX) for the indicated time periods. NLRP3 expression was quantitated by measuring band intensities using the “ImageJ” software. The values were normalized to Actin. Data are represented as mean ± SD (two-tailed t test WT vs. Trim28CKO, ***p = 1 × 10−12, 3 × 10−17). f Immunoblot analysis of NLRP3 expression in mouse PMs from WT or Trim28CKO mice stimulated with LPS for 4 h, together with DMSO or MG132 (10 μM) treatment for 4 h. g Immunoblot analysis of NLRP3 expression in mouse PMs from WT or Trim28CKO mice stimulated with LPS for 4 h, together with DMSO or chloroquine (50 μM) treatment for 4 h. Similar results were obtained from three independent experiments.