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. 2021 Aug 10;7:64. doi: 10.1038/s41421-021-00300-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Pseudotime trajectories for CD8⁺ T cells based on Monocle2, color-coded for the CD8⁺ T cell phenotypes. b Pseudotime trajectories for CD8⁺ T cells, color-coded for the pseudotime. c Gene expression dynamics along the CD8⁺ T cells lineage. Genes were clustered into 6 gene sets, and each of them was characterized by specific expression profiles. d Genes involved in the function and response of T cells modeled along the CD8⁺ T cell lineages at different timepoints. See also “Materials and methods” for constructing single-cell trajectories.