Fig. 6.

Raw cells were stimulated with or without CSE for 24 h. The supernatant of Raw cells taking four kinds of stimulations (control, CSE, glutamine abundance, and deprivation) was collected. The Leuk-1 cells were cultured in the supernatant of Raw. CCK-8 results are shown in a. Migration ability of Leuk-1 cells were analyzed using a wound-healing assay in the presence of Raw cells supernatant (b, Fig. S1). The apoptosis ratios are shown in panels c and d which were detected by flow cytometry with Annexin V- FITC/PI double staining. Error bars, SEM. *P < 0.05, **P < 0.01, ***P < 0.001