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. 2021 Aug 9;12(8):780. doi: 10.1038/s41419-021-04034-7

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© The Author(s) 2021, corrected publication 2021

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Fig. 1 — a Relative mRNA level of Fanci in various tissues. Data are presented as mean ± SD. b Western blot analysis of the wild type and Fanci-flag testes (8 weeks old). α-Tubulin was used as a loading control. c Expression and localization of FANCI in adult testes. Immunofluorescence analysis of FLAG was performed on paraffin sections from Fanci-flag testes. The nuclei were stained with DAPI. Arrowhead represents spermatogonia. Arrow represents spermatocyte. Scale bars, 20 μm. d Localization of FANCI in chromosome spread of Fanci-flag spermatocytes. Meiotic chromosome spreads were immunolabeled with anti-FLAG and anti-SYCP3 antibodies. Scale bars, 5 μm.