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. 2021 Aug 9;12(8):780. doi: 10.1038/s41419-021-04034-7

Fig. 2. FANCI deletion results in atrophytestes and male sterility.

Fig. 2

a Western blot analysis of the wild type and Fanci−/− testes (8 weeks old). α-Tubulin was used as a loading control. b The mRNA probe detection of wild type and Fanci−/− testes. Scale bars, 20 μm. c Representative images of the testes of wild-type and Fanci−/− mice. d Body weights of wild-type and Fanci−/− mice at 8 weeks after birth. Six mice were analyzed for each group, and data are presented as mean ± SD. *, P < 0.05. Student’s t-test. e Mean ratio of testis/body weight from 8 weeks old wild type and Fanci−/− mice. Six mice were analyzed for each group, and data are presented as mean ± SD. *, P < 0.05. Student’s t-test. f Fertility test result of the mean ratio of pups/litter from wild type and Fanci−/− mice. Six wild-type mice: 34 litters and 12.8 pups/litter. Six Fanci−/− mice: 0 litter. Data are presented as mean ± SD. *, P < 0.05. Student’s t-test.