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. 2021 Aug 9;12(8):780. doi: 10.1038/s41419-021-04034-7

Fig. 4. FANCI deletion increases apoptosis of germ cells and affects the maintenance of undifferentiated spermatogonia.

Fig. 4

a TUNEL assay of testes from 8-week-old wild-type and Fanci−/− mice. Cells stained red are TUNEL-positive. Scale bars, 20 μm. b Frequencies of TUNEL-positive tubules in wild type and Fanci−/− mice. In each group, six mice were analyzed. Data are presented as mean ± SD. *, Chi-square test (Fisher’s exact test). c Numbers of TUNEL-positive cells per TUNEL-positive tubule in wild type and Fanci−/− mice. Six wild-type mice and six Fanci−/− mice were analyzed. Data are presented as mean ± SD. *, P < 0.05. Student’s t-test. d Immunohistochemistry staining against PLZF (an undifferentiated spermatogonia marker) on wild type and Fanci−/− testes at 8 weeks old and at 12 months old. Arrows represent the undifferentiated spermatogonia. Scale bars, 50 μm. Scoring of the percentage of PLZF-positive tubules per total tubule (e) and scoring of the number of PLZF-positive cells per PLZF-positive tubule (f). A total of 100 tubules from three independent mice were examined at 8 weeks and 12 months, both for wild type and Fanci−/− mice. In each group, six mice were analyzed. Data are presented as mean ± SD. *, P < 0.05. Ns no statistical significant difference. Frequencies were compared by the Chi-square test (Fisher’s exact test). Numbers were compared by Student’s t-test.