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. 2021 Aug 9;12(8):780. doi: 10.1038/s41419-021-04034-7

Fig. 6. Meiotic prophase I stage progression and synapsis in Fanci−/− spermatocytes.

Fig. 6

a Wild type and Fanci−/− spermatocytes were immunolabeled with SYCP1 and SYCP3 in leptotene (Lep), zygotene (Zyg), pachytene (Pac), and diplotene (Dip) spermatocytes. Meiotic stages were determined on the basis of SYCP3 signals. b Percentage of each stage of meiotic prophase as determined by SYCP3 immunostaining. In each group, six mice were analyzed. Data are presented as mean ± SD. Ns no statistical significant difference. Chi-square test (Fisher’s exact test). c Wild type and Fanci−/− spermatocytes were immunolabeled with γ-H2AX and SYCP3 at leptotene (Lep), zygotene (Zyg), pachytene (Pac), and diplotene (Dip) spermatocytes. d Spermatocytes immunolabeled for RNA Polymerase II (RNA Pol II) and SYCP3 at diplotene spermatocytes. Arrows represent the sex chromosomes. Scale bars, 5 μm.