Figure 2.

Repeated injection of PD fluid leads to a progressive change in MΦres phenotype. C57BL/6 mice were injected with PD fluid (filled circle) or left untreated (open square) 5 times a week for the indicated number of injections. At each timepoint whole PEC were isolated 24 h after the last injection and analysed by flow cytometry. (A) Schematic depiction of experimental timeline. (B–D) Number of MΦres, MΦmono and Ly6C high monocytes. (E–H) Expression of MΦres associated cell-surface markers on F4/80 high MΦ. (I) Intracellular expression of Gata6 in MΦres identified in (A). Datapoints depict individual animals and lines indicate (B–H) median or (I) mean with SD. Data from a single experiment using 3 animals per group per timepoint. Data analysed using 2-way ANOVA followed by Tukey’s post-hoc test after transformation. n.s., not significant; *p < 0.05; **p < 0.01; ***p<0.001; ****p < 0.0001.