Figure 5.

MΦres from PD fluid-injected animals show progressively enhanced responses to stimulation. C57BL/6 mice were injected with PD fluid (circles) or left untreated (squares) 5 times a week for the indicated number of injections. At each timepoint whole PEC were isolated 24 h after the last injection and incubated in vitro in the presence of pHrodo labelled apoptotic thymocytes for 90 minutes (A, B) or stimulated with LPS/IFNγ (C, D; 6 h) or recombinant IL-4 (E, F; 24 h) as a control non-stimulated cells (open squares) were used. Uptake of apoptotic cells by total CD11b+ myeloid cells (A) or MΦres (CD102+I-A/I-E low) (B) as well as expression of MΦres associated cell-surface markers (C–F) assessed by flow cytometry. Datapoints depict individual animals and lines indicate mean and SD. Data from a single experiment. Data analysed using 2-way ANOVA followed by Tukey’s post-hoc test after transformation. n.s., not significant; *p < 0.05; **p < 0.01; ***p<0.001; ****p < 0.0001.