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. 2021 Jul 27;12:700637. doi: 10.3389/fmicb.2021.700637

FIGURE 5.

FIGURE 5

Comparison between the family-level taxonomic composition of the bacterial community structure obtained by pyrosequencing and the composition of the bacterial isolates cultured by HTC. (A) Seasonal variation of the bacterial community structure at the family level observed at 1 and 50 m depths in Lake Soyang, as characterized by 16S rRNA gene pyrosequencing. (B) Phylogenetic composition and abundance of 605 axenic HTC isolates at the family level. The bubbles represent the percentage of pyrotags of each taxonomic group in each sample. Taxonomic groups with gray shades represent major phylogenetic groups that occupied > 1% of the total bacterial community in eight samples or > 2.5% of the bacterial community in each sample. Taxonomic classification of 16S rRNA gene sequences of HTC isolates and pyrotags was determined using the Mothur software based on the SILVA taxonomy database. a Contains the acIV-A, -B, and -C lineages. b Contains acIV-D lineage. c Contains the acI lineage.