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. 2021 Jul 27;12:700637. doi: 10.3389/fmicb.2021.700637

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Copyright © 2021 Kim, Islam, Kang and Cho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison between the composition of HTC isolates and pyrotags at the tribe level belonging to the acI and acIV lineages of the phylum Actinobacteria. (A) Seasonal variation of tribes of the acI and acIV lineages, observed at 1 and 50 m depths in Lake Soyang, as characterized by 16S rRNA gene pyrosequencing. (B) Number of HTC isolates belonging to tribes of the acI and acIV linages. The bubbles represent the percentage of pyrotags of each taxonomic group in each sample. Taxonomic classification of 16S rRNA gene sequences of HTC isolates and pyrotags was determined using the freshwater-specific FreshTrain database (Rohwer et al., 2018).