Figure 3.
Donor- and host-derived reparative genes in the rat heart after hAM-MSC-dressing therapy
The rat ICM hearts were explanted at 3 days after transplantation of 2 × 106 hAM-MSCs by the-dressing method. gDNA was extracted from 25 mg of LV tissue, and 100 ng DNA was amplified by qPCR. (A) Ratio of human and rat DNA contained in the xenogeneic sample. Mean ALU quantification cycle (Cq) values were used to calculate the ratio of human gDNA in each heart gDNA sample according to a 10-fold dilution series of known ratios of rat heart gDNA and hAM-MSC gDNA. n = 5 with 3 technical replicates. (B) The number of gene transcript copies in 10 ng cDNA isolated from the hAM-MSC-dressing group. Expression levels of human and rat reparative genes were determined in ischemic LV tissue of the hAM-MSC-dressing group utilizing gDNA standard curves of 1 × 107–1 × 102 gene copies and species-specific primer pairs (human versus rat) in the xenogeneic sample. Data points presented as mean (log10). n = 5 with 3 technical replicates. ∗∗p < 0.01 and ∗∗∗p < 0.001. Student’s t test.