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. 2021 Aug 3;148(15):dev191619. doi: 10.1242/dev.191619

Fig. 3.

Fig. 3.

Taf15 depletion by translation-blocking MO leads to single-intron retention in fgfr4 in Xenopus tropicalis embryos. (A) Visualization of fgfr4 RNA-seq reads with IGV aligned with the gene model in blue. Red bars indicate qRT-PCR primers used to measure retained introns. Orange bars indicate qRT-PCR primers used to measure total transcripts. (B) qRT-PCR for fgfr4 intron 1 expression in stage-15 embryos. Intron expression levels are normalized to total fgfr4 expression. ***P<0.0001. (C) qRT-PCR for total fgfr4 expression in stage-15 embryos. **P<0.005, ***P<0.001, ****P<0.0001. In B,C, embryos treated as indicated on the x-axes; y-axes show fgfr4 expression relative to eef1a1 and normalized to uninjected embryos. Data are mean±s.d.; n=9 individual embryos. All means were compared by one-way ANOVA followed by Tukey post-hoc analyses. All injections for downstream qRT-PCR analysis were into both cells of two-cell-stage embryos. MO, M+Z-Taf15-depleting MO; UC, uninjected control.