FIGURE 3.

DIlp7 regulates food intake, transition and absorption of adult flies kept on yeast-free food. (A) Shown are box-plots depicting survival rates of staged adult flies transferred onto yeast-free corn food (CF) at 25°C. Plotted is time (in days, d) to reach 50% lethality for genetic controls (mCherry:FOXO, Cntr) and different dIlp mutants as indicated. One black dot = one experiment. Significance calculated by Mann-Whitney’s test, Cntr vs. ΔdIlp², ΔdIlp^2–3: p = 0.0286^∗; Cntr vs. ΔdIlp³: p = 0.5714^ns; Cntr vs. ΔdIlp⁷, ΔdIlp^2,7: p = 0.0159^∗. For crude data, see Supporting informations_Raw data_Main figures/Supplementary Figure 3A. (B,C) Plotted are the percentage of adult females kept on dye-containing corn food (CF) with colored abdomens after 4 h (B), and colored-abdomen flies transferred onto dye-less corn food (CF) with colored abdomens after 4 h (C) at 20°C. The data for genetic controls (mCherry:FOXO, Cntr) and different dIlp mutants are depicted as box plots; one black dot = one experiment. (B) Significance calculated by Kruskal-Wallis test, Cntr vs. ΔdIlp²: p = 0.0282^∗; Cntr vs. ΔdIlp⁷: p = 0.0132^∗; Cntr vs. ΔdIlp^2–3: p > 0.9999^ns; Cntr vs. ΔdIlp^2,7: p = 0.013^∗∗. For crude data, see Supporting informations_Raw data_Main figures/Supplementary Figure 3B. (C) Significance calculated by Kruskal-Wallis test, Cntr vs. ΔdIlp²: p = 0.2396^ns; Cntr vs. ΔdIlp⁷: p = 0.0016^∗∗; Cntr vs. ΔdIlp^2–3: p > 0.9999^ns; Cntr vs. ΔdIlp^2,7: p = 0.0012^∗∗. For crude data, see Supporting informations_Raw data_Main figures/Supplementary Figure 3C. (D) Starved flies were transferred onto plates loaded with protein baits at 20°C and hemolymph was taken after set time intervals (in hours, h; 0, 1, and 2 h). Plotted are measured hemolymph protein levels of genetic controls (mCherry:FOXO, Cntr) and different dIlp mutants as indicated. Error bars represent standard deviation; plotted are the mean. Significances calculated by Dunnett’s multiple comparison test but not shown to avoid overloaded graph, 0 h: Cntr vs. ΔdIlp²: p = 0.072^∗∗; Cntr vs. ΔdIlp⁷: p = 0.9132^ns; Cntr vs. ΔdIlp^2–3: p = 0.4179^ns; Cntr vs. ΔdIlp^2,7: p = 0.8744^ns. 1 h: Cntr vs. ΔdIlp²: p = 0.9990^ns; Cntr vs. ΔdIlp⁷: p = 0.4465 ns; Cntr vs. ΔdIlp^2–3: p = 0.0002^∗∗∗; Cntr vs. ΔdIlp^2,7: p = 0.8939^ns. 2 h: Cntr vs. ΔdIlp²: p < 0.0001^****; Cntr vs. ΔdIlp⁷: p = 0.0260^∗; Cntr vs. ΔdIlp^2–3: p = 0.0285^∗; Cntr vs. ΔdIlp^2,7: p = 0.047^∗∗. For crude data, see Supporting informations_Raw data_Main figures/Supplementary Figure 3D.