FIGURE 4.

DIlp7 regulates systemic Insulin signaling in adult fed on yeast-free plant diet. (A,B) Adult head samples were probed with antibodies for phosphorylated AKT^85–Ser505 and total AKT amounts (pan-AKT). Shown are western blot photographs from genetic control (mCherry:FOXO, Cntr) and different dIlp mutants kept on corn food (CF) at 20°C. AKT^85–Ser505 / pan-AKT signal ratios were normalized to controls and plotted as AKT⁵⁰⁵ activity (B). Genetic controls (mCherry:FOXO, Cntr), different dIlp mutants and flies with Lgr3 knocked-down in IPCs (Lgr3^KD) were measured and plotted on box-plots. The activity of each genotype was normalized to the control (Cntr, gray, median = 100%). Significances not shown if the n is not enough significant (n < 3). Statistics, Kruskal-Wallis test, p > 0.05^ns; p = 0.0145^∗. For crude data, see Supporting informations_Raw data_Main figures/Supplementary Figure 4B.